Fishing for function: zebrafish BAC transgenics for functional genomics
نویسندگان
چکیده
منابع مشابه
Fishing for function in zebrafish.
Fishing for Function in Zebrafish Print ISSN: 0009-7330. Online ISSN: 1524-4571 Copyright © 2006 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Circulation Research doi: 10.1161/01.RES.0000217601.30361.b1 2006;98:723-726 Circ Res. http://circres.ahajournals.org/content/98/6/723 World Wide Web at: The...
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We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to great...
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Stefan Schulte-Merker has been working with zebra®sh for over ten years and was involved in a number of genetic screens. He was also part of a team that produced a ®rst-generation map of the `Goodfellow panel' of radiation hybrids, which was intended to help in the cloning of the mutated genes. He is currently Head of Fish Genetics at Artemis Pharmaceuticals, in TuÈbingen, who are in the proces...
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In the post genome era, a major goal in molecular biology is to determine the function of the many thousands of genes present in the vertebrate genome. The zebrafish (Danio rerio) provides an almost ideal genetic model to identify the biological roles of these novel genes, in part because their embryos are transparent and develop rapidly. The zebrafish has many advantages over mouse for genome-...
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Since the completion of the human genome sequencing project, morpholino phosphorodiamidate oligonucleotide (MO) knockdown in zebrafish has been increasingly used to elucidate human gene function. As part of the effort to expand the functional genomics screening capacity at the University of Wisconsin-Stout, this project investigated MO microinjection techniques, embryo production, and brine shr...
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ژورنال
عنوان ژورنال: Molecular BioSystems
سال: 2011
ISSN: 1742-206X,1742-2051
DOI: 10.1039/c1mb05116d